Composite

Part:BBa_K1462340:Design

Designed by: Ye Peng   Group: iGEM14_SCUT   (2014-10-10)


pTDH3+RuBisCo+SH3-ligand+tADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This recombinant consists of four parts, the constitutive promoter TDH3, the key enzyme RuBisCo, the ligand of the scaffold SH3 and, the teminator ADH1.



Source

We got pTDH3 and tADH1 from kits, RuBisCo from a synthesis company and SH3-ligand by PCR(included in the reverse primer).


References